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Rf value of eugenol

Rf value of eugenol

rf value of eugenol

E-mail: aneesahmedkhalil gmail. Eugenol, a phytogenic bioactive component is frequently found in diversified herbal plants possessing well-defined functional attributes.

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Prominent sources of eugenol are clove, cinnamon, tulsi and pepper. Various extraction methods have been practiced globally for the extraction of eugenol and other nutraceutics from plants. The most extensively employed approaches in this regard include solvent extraction, hydro-distillation, microwave-assisted extraction, supercritical carbon dioxide extraction and ultrasound-based extraction. Eugenol has been approved to encompass numerous beneficial aspects against a capacious spectrum of life threatening indispositions including oxidative stress, inflammation, hyperglycemia, elevated cholesterol level, neural disorders and cancer.

In addition, eugenol has also shown strong potential as an antimicrobial agent against wide ranges of pathogenic and spoilage causing microorganisms.

Consequently, this article is an attempt to elucidate the general properties, sources, extraction methods, therapeutic role and associated mechanisms of eugenol. Dr Anees Ahmed Khalil is a fresh Ph. His research interests are therapeutic and nutraceutical properties of fruits and vegetables.

He has done his Ph. Currently, he is the author of 4 impacted international research publications. Ubaid ur Rahman is a Ph. Currently he is working on exploring the potential of various spectroscopic and molecular approaches for predicting meat quality and microbial safety.

He has authored 9 international impacted research and review articles, four international book chapters and 34 conference papers. His research domain is food product development and functional properties of food commodities. His main area of focus is fruits and vegetables processing with special reference to value addition, waste management and formulation of functional fruit products. He also has published a number of research and review articles. She has a number of impacted publications having international repute.

She has produced five MSc students in food technology as main supervisor. Currently, she is supervising 2 Ph. Tariq Mehmood is a Ph.

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He is currently working on utilization of citrus waste as functional fruit leather. Muneeb Khan is a Ph. His research project is assessing authenticity of meat and meat products by applying spectroscopic and molecular techniques. Eugenol is a phenolic component that can be obtained from a wide range of plant sources including clove oil, nutmeg oil, cinnamon extract and many other plants. It owns strong health promoting functions that make it a versatile natural ingredient.

Eugenol was firstly extracted from the leaves and buds of Eugenia caryophyllata commonly named as clove. Currently, eugenol can also be synthesized by allylation of guaiacol with allyl chloride having the similar kind of functional property. Eugenol is present in significant amount in the extracts of numerous medicinal herbs so it has fascinated the attention of several researchers and opened up the gateway of research regarding its utilization as a medicine to cure various diseases.

Eugenol is avowed to possess certain pharmacological properties including anaesthetic activity, antioxidant potential, antimicrobial role, anti-inflammatory action, anti-carcinogenic effects, neuroprotective ability, hypolipidemic efficiency and anti-diabetic effectiveness.

The general physical and chemical properties of eugenol are discussed in Table 1. As a result of a wide spectrum of biological and functional properties, eugenol is still proclaimed as the priority of research.

Therefore, it is of significant worth to rationally confederate the research findings related to the therapeutic potential of eugenol to elucidate its importance for human health and mechanisms involved in the functionality of eugenol to obviate several lifestyle related indispositions. Several modifications have been made in the conventional solvent extraction process, which show higher efficiency as compared to the traditional method.

As an instance, batch extraction process is an attractive alternative to the Soxhlet extraction.All Rights Reserved. The material on this site can not be reproduced, distributed, transmitted, cached or otherwise used, except with prior written permission of Multiply. Hottest Questions. Previously Viewed.

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rf value of eugenol

Botany or Plant Biology. Wiki User Every compound has a specific Rf value for every specific solvent and solvent concentration and can be helpful in identifying unknown samples with Rf values of known compounds.

RF value is the distance travelled by a component of a mixture relative to the solvent.

Isolation and Purification of Eugenol from Cloves

That is, it is the distance travelled by the component divided by the distance travelled by the solvent. RF value for any component has a fixed value less than unity. By calculating RF value for a component and comparing it with known values, a given component can be identified. Asked in School Subjects Does the Rf value of a pigment stay the same if a different pigment is used?

Yes, It does if the same colour is used. If the colour changes, the Rf value changes. Hope this was useful XxX.

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The Rf value for aspirin is 1. For ibuprofen, the Rf value is 1. Rf stands for retardation factor. Rf value is an indicator of the solubility of a pigment.

rf value of eugenol

Identical molecules means of two pigments has the same Rf value. Small Rf value means less soluble and pigments with Rf value near to one means highly soluble. Asked in Electronics Engineering Is an rf power amplifier useful in the home?

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The answer to whether an RF power amplifier is useful for your home depends on the qualifty if your equipment.The Rf values are equal for the three spots, indicating high purity of the eugenol product extracted in the earlier steps, as its Rf value is equal to that of the standard eugenol solution.

The co-spot confirms that the spots in lanes 1 and 3 have migrated an equal distance, as there is only one spot comprising both the standard eugenol solution and the eugenol extract in lane 2, as opposed to two spots which would indicate that the standard eugenol solution spot and the product spot are in fact distinct and separate, and only appear to have migrated the same distance due to parallax error. Thus our product is a highly pure extract of eugenol. The spots are relatively large, indicating that the original volume spotted at the baseline may be too large.

The co-spot is especially large and unevenly shaped, due to the greater combined volumes of the standard eugenol solution and product spotted at lane 2.

The accuracy can be improved by spotting a smaller volume initially. Post-Lab Questions 1. Eugenol dissolves better in DCM.

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Eugenol is a largely non-polar molecule. Although it has a highly polar hydroxyl group and a polar ether group, most of its bonds are non-polar carboncarbon or carbon-hydrogen bonds, and it contains a large non-polar benzene ring. Water molecules are highly polar due to the highly electronegative O-H bond, and because the bond dipoles do not cancel out due to the bent geometry of the molecule. Although water is able to form strong hydrogen bonds with the hydroxyl and ether groups of eugenol, it only interacts weakly with the remaining non-polar parts of the eugenol molecule via weak London Dispersion Forces LDF and dipole-induced dipole forces.

The intermolecular forces IMF between water and eugenol molecules are weaker than those between water molecules and those between eugenol molecules, thus eugenol is not very soluble in water. Dichloromethane DCMlike eugenol, is only slightly polar. It has two electronegative C-Cl bonds, and two non-polar C-H bonds. The polar bonds in DCM forms strong dipole-dipole forces DDF with the polar hydroxyl and ether groups in eugenol; at the same time, DCM also has nonpolar C-H bonds that interact well and form LDF with the non-polar C-H bonds in eugenol, and, in particular, eugenols large non-polar benzene ring.

The intermolecular forces between DCM and eugenol molecules are stronger than the IMF between water and eugenol molecules, thus eugenol is more soluble in DCM than in water. It is more efficient to use several small portions rather than one large portion of solvent to extract a sample. The concentration of A eugenol remaining in an aqueous solution the distillate containing the eugenol oil dispersed in water after i extractions with an organic solvent DCM is given by.

As K must be positive, and is smaller for the three-extractions-usingmL for all positive values of K, the three extractions always imparts greater extraction efficiency, extracting a higher concentration of eugenol into the DCM and leaving a smaller concentration remaining in the distillate.

NaOH reacts with eugenol in the organic phase, forming a water-soluble salt sodium eugenate:. The eugenate anion dissolves into the aqueous phase because the negatively charged ion can form strong ion-dipole forces with polar water molecules, which are stronger than the intermolecular forces between the eugenate ions and between the water molecules. This is a step in purifying eugenol from the distillate, as we are extracting eugenate anions into the aqueous phase, leaving impurities behind to be discarded together with the organic phase.

The eugenate ions can then be converted back to eugenol by acidifying the aqueous phase, thus protonating the anions:. Litmus paper cannot be used instead. The pH range of Congo red paper is 3. HCl added to the aqueous solution protonates the eugenate anions, converting them back to eugenol molecule.In chromatographythe retardation factor R is the fraction of an analyte in the mobile phase of a chromatographic system.

Although the term retention factor is sometimes used synonymously with retardation factor in regard to planar chromatography the term is not defined in this context.

However, in column chromatographythe retention factor or capacity factor k is defined as the ratio of time an analyte is retained in the stationary phase to the time it is retained in the mobile phase, [3] which is inversely proportional to the retardation factor. In chromatography, the retardation factor, Ris the fraction of the sample in the mobile phase at equilibrium, defined as: [1]. The retardation factor, R fis commonly used in paper chromatography and thin layer chromatography for analyzing and comparing different substances.

It can be mathematically described by the following ratio: [2]. An R f value will always be in the range 0 to 1; if the substance moves, it can only move in the direction of the solvent flow, and cannot move faster than the solvent.

For example, if particular substance in an unknown mixture travels 2. One can choose a mobile phase with different characteristics particularly polarity in order to control how far the substance being investigated migrates.

An R f value is characteristic for any given compound provided that the same stationary and mobile phases are used. It can provide corroborative evidence as to the identity of a compound. If the identity of a compound is suspected but not yet proven, an authentic sample of the compound, or standard, is spotted and run on a TLC plate side by side or on top of each other with the compound in question. Note that this identity check must be performed on a single plate, because it is difficult to duplicate all the factors which influence R f exactly from experiment to experiment.

From Wikipedia, the free encyclopedia. Affinity chromatography Column chromatography Displacement chromatography Electrochromatography Gas chromatography High-performance liquid chromatography Capillary electrochromatography Ion chromatography Micellar electrokinetic chromatography Normal-phase chromatography Paper chromatography Reversed-phase chromatography Size-exclusion chromatography Thin-layer chromatography Two-dimensional chromatography.

Steam Distillation of Eugenol Essay

Gas chromatography—mass spectrometry Liquid chromatography—mass spectrometry Pyrolysis—gas chromatography—mass spectrometry. Distribution constant Freundlich equation Kovats retention index Retention factor Van Deemter equation. Category Commons Analytical Chemistry. Categories : Chromatography.

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Namespaces Article Talk. Views Read Edit View history. By using this site, you agree to the Terms of Use and Privacy Policy.The current Linnean nomenclature term for cloves is Syzygium aromaticum. Eugenol is used in perfumesflavoringsand essential oils. It is also used as a local antiseptic and anaesthetic. For persons with a dry socket as a complication of tooth extraction, packing the dry socket with a eugenol-zinc oxide paste on iodoform gauze is effective for reducing acute pain.

It is one of many compounds that is attractive to males of various species of orchid beeswhich apparently gather the chemical to synthesize pheromones ; it is commonly used as bait to attract and collect these bees for study. Clove oil is growing in popularity as an anaesthetic for use on aquarium fish as well as on wild fish when sampled for research and management purposes.

The biosynthesis of eugenol begins with the amino acid tyrosine. L -tyrosine is converted to p -coumaric acid by the enzyme tyrosine ammonia lyase TAL. Coniferaldeyhyde is then further reduced to coniferyl alcohol by cinnamyl-alcohol dehydrogenase CAD or sinapyl-alcohol dehydrogenase SAD.

Finally, coniferyl acetate is converted to eugenol via the enzyme eugenol synthase 1 and the use of NADPH. Eugenol and thymol were found to possess general anesthetic properties. Like many other anesthetic agents, these 2-alkyl oxy phenols were found to act as positive allosteric modulators of the GABA A receptor.

Although these eugenol and thymol are too toxic and not potent enough to use clinically, these findings led to the development of 2-substituted phenol anesthetic drugs, including propanidid later withdrawn and the widely used propofol.

Eugenol is hepatotoxicmeaning it may cause damage to the liver. Eugenol is subject to restrictions on its use in perfumery [27] as some people may become sensitised to it, however, the degree to which eugenol can cause an allergic reaction in humans is disputed. Eugenol is a component of balsam of Peruto which some people are allergic.

From Wikipedia, the free encyclopedia. CAS Number. Interactive image.

Retardation factor

PubChem CID. Chemical formula. Cloves Syzygium aromaticum [31] [32] [33] Wormwood [ citation needed ] Cinnamon [32] [34] Cinnamomum tamala [35] Nutmeg Myristica fragrans [36] Ocimum basilicum sweet basil [37] Ocimum gratissimum African basil [16] [38] Ocimum tenuiflorum syn.During thin layer chromatography, a technician places spots of a chemical mixture on a flat plate coated with a layer of absorbent material, then sits the lower portion of the plate in solvent.

As solvent moves up the plate, chemicals in the mixture move up with it at different rates, so they separate.

Oil of Cloves: The Benefits of Eugenol

This test determines which chemicals are in a mixture by comparing the travel rates of unknowns to known standards. The results, however, can be affected by many factors. Retention factor values in thin layer chromatography are affected by the absorbent, the solvent, the chromatography plate itself, application technique and the temperature of the solvent and plate. The retention factor for a chemical during thin layer chromatography is a measure of how far it moves up the plate in response to the solvent movement.

Since the absolute movement of the chemical depends on how far the solvent travels, you calculate retention factor values relative to the degree of solvent movement. The retention factor for a chemical is the vertical distance moved by the chemical from the spot where it was originally applied to the plate, divided by the distance traveled by the solvent — measured from the same starting point.

The thin layer chromatography plate itself can affect the retention factor value obtained for a given chemical. Thin layer chromatography plates can be coated with a variety of absorbent solids; most frequently silica or alumina. Since the retention factor is based on the relative affinity of the chemical for the absorbent compared to the solvent, changing the absorbent can greatly change the retention factor.

Also, the thickness and uniformity of the layer of absorbent can vary from plate to plate, especially if they are handmade. These factors can also change the retention factor value for chemicals. Since the solvent carries the chemical up the plate, the particular solvent used will also have a substantial impact on the retention factor value for the chemical. A solvent which has a stronger interaction for a particular chemical will more easily overcome any affinity of the chemical for the absorbent layer, and move that chemical farther in a given period of time.

Mixtures of solvents can also have different effects depending on the proportion of each solvent. There are a few other factors which may affect the retention factor in some cases. The temperature of the solvent and plate may make slight changes, since, for example, the solvent can often better dissolve the chemicals it is transporting at higher temperatures.

The technique of the technician in applying the sample to the plate may also change the retention factor. Applying too much sample may result in large, diffuse bands of chemical moving up the plate, making it difficult to accurately measure the distance the chemical has been transported.

Michael Judge has been writing for over a decade and has been published in "The Globe and Mail" Canada's national newspaper and the U. Michael has worked for an aerospace firm where he was in charge of rocket propellant formulation and is now a college instructor. TL;DR Too Long; Didn't Read Retention factor values in thin layer chromatography are affected by the absorbent, the solvent, the chromatography plate itself, application technique and the temperature of the solvent and plate.We use cookies to give you the best experience possible.

Introduction: This experiment involves an extraction of a natural product using the techniques of steam distillation. The principle component of oil of cloves is an aromatic compound, which is identified by thin layer chromatography TLC.

Eugenol is widely used in dentistry, due to its analgesic, antiseptic balsamic qualities. It is ideal for curative for pulp hyperemia the soft, sensitive substance underneath the enamel and dentine of a tooth and pulp its. In dentistry eugenol is also used to cover cavities, fill canals, etc. Don't use plagiarized sources. It also keeps food fresh and kills off bacteria and viruses. Eugenol helps keep meat fresh and prevents stomach upset, diarrhea, and wound infections.

In addition, it might play a part in relieving indigestion, controlling diarrhea, and healing cuts and bites. In a mL round bottom flask, place Set up for a simple distillation. Heat to boiling and collect the distillate until you no longer see droplets of organic liquid coming out of the condenser about 40 minutes. Place the distillate in a separatory funnel and extract with three 10 mL portions of Dichloromethane. Combine the dichloromethane extracts and dry over Sodium Sulfate.

Examine the purity of the extract using thin-layer chromatography TLC. Spot a small sample of the extract using a capillary spotter about 1 cm from the end of TLC plate. Visualize using a UV light and mark the visualized spots with a pencil. While the plate is developing set up a clean and dry distillation set up and distil off most of the dichloromethane solvent. The remaining solution of Eugenol in Dichloromethane can be removed using a disposable pipette and placed into a clean pre-weighted vial.

The vial can be heated on a steam bath under the hood to remove the small amount of dichloromethane.

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The pure Eugenol should be weighted to determine the percent oil recovered based on the mass of cloves originally used.